Aconitase 2 Recombinant Rabbit Monoclonal Antibody [JE40-83] (ET7109-15)
Catalog# ET7109-15
Rabbit Monoclonal to Aconitase 2
Tested for
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WB
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IF-Cell
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IHC-P
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IP
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Aconitase 2 on different lysates with Rabbit anti-Aconitase 2 antibody (ET7109-15) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: L6 cell lysate
Lane 4: Mouse small intestine tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: L6 cell lysate
Lane 4: Mouse small intestine tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
