Cellular Apoptosis Susceptibility Recombinant Rabbit Monoclonal Antibody [JU34-33] (ET7106-90)
Catalog# ET7106-90
Rabbit Monoclonal to Cellular Apoptosis Susceptibility
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cellular Apoptosis Susceptibility on different lysates with Rabbit anti-Cellular Apoptosis Susceptibility antibody (ET7106-90) at 1/1,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: SiHa cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Ramos cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 110/50 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-90) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: SiHa cell lysate (20 µg/Lane)
Lane 5: PC-3M cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Ramos cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 110/50 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-90) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.