CRM1 Recombinant Rabbit Monoclonal Antibody [JB35-22] (ET7107-27)
Catalog# ET7107-27
Rabbit Monoclonal to CRM1
Tested for
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CRM1 on different lysates with Rabbit anti-CRM1 antibody (ET7107-27) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.