OGT Recombinant Rabbit Monoclonal Antibody [JB44-39] (ET7107-17)

UNIPROT #

O15294

PROTEIN NAME

OGT

SYNONYMS

FLJ23071 antibody;GlcNAc transferase antibody;HRNT1 antibody;MGC22921 antibody;O GlcNAc antibody;O GlcNAc transferase p110 subunit antibody;O GlcNAc transferase subunit p110 antibody;O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody;O linked N acetylglucosamine (GlcNAc) transferase antibody;O linked N acetylglucosamine transferase 110 kDa subunit antibody;O-GlcNAc transferase subunit p110 antibody;O-linked N-acetylglucosamine transferase 110 kDa subunit antibody;ogt antibody;OGT1_HUMAN antibody;UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody;UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody;UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody;UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody;Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody

SEQUENCE SIMILARITIES

Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.

TISSUE SPECIFICITY

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its proteasomal degradation.; Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Membrane.

FUNCTION

O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.