Albumin Recombinant Rabbit Monoclonal Antibody [JF32-10] (ET1702-55)
Catalog# ET1702-55
Rabbit Monoclonal to Albumin
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WB
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IHC-P
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mIHC
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IF-Tissue
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Human
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Mouse
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Rat
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Cow
Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-55) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Albumin Recombinant Rabbit Monoclonal Antibody [JF32-10] (ET1702-55)
Immunogen
Synthetic peptide within human albumin aa 156-189 / 609.
Host
Rabbit
Positive Control
Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse spleen tissue lysate, human lung tissue, human liver tissue, human kidney tissue, mouse liver tissue, rat liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF32-10
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:40,000
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IHC-P
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1:1,000-1:30,000
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mIHC
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1:3,000
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IF-Tissue
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 69 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Albumin
SYNONYMS
alb antibody;ALBU_HUMAN antibody;Albumin (32 AA) antibody;Albumin (AA 34) antibody;cell growth inhibiting protein 42 antibody;DKFZp779N1935 antibody;GIG20 antibody;GIG42 antibody;growth-inhibiting protein 20 antibody;OTTHUMP00000220436 antibody;OTTHUMP00000220438 antibody;OTTHUMP00000220439 antibody;PRO0883 antibody;PRO0903 antibody;PRO1341 antibody;PRO1708 antibody;PRO2044 antibody;PRO2619 antibody;PRO2675 antibody;Serum albumin antibody;UNQ696/PRO1341 antibody
SEQUENCE SIMILARITIES
Belongs to the ALB/AFP/VDB family.
TISSUE SPECIFICITY
Plasma.
POST-TRANSLATIONAL MODIFICATION
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.; Glycated in diabetic patients.; Phosphorylated by FAM20C in the extracellular medium.; Acetylated on Lys-223 by acetylsalicylic acid.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Serum albumin (ALB), the main protein in plasma, has a very good binding capacity for water, fatty acids, calcium, sodium, bilirubin, hormones, potassium and drugs. The primary function of ALB is to regulate the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted form of albumin. Mutations in the ALB gene may result in familial dysalbuminemic hyperthyroxinemia (FDH), a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T4. FDH is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian populations.
CITATIONS
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Mei, Y., Li, M., Wen, J., Kong, X., & Li, J.
Single-cell characteristics and malignancy regulation of alpha-fetoprotein-producing gastric cancer
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WB
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Human
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Citation
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Droplet-engineered organoids recapitulate parental tissue transcriptome with inter-organoid homogeneity and inter-tumor cell heterogeneity
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IF
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Mouse
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Citation
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