Catalase Recombinant Rabbit Monoclonal Antibody [JM11-12] (ET1703-31)
Catalog# ET1703-31
Rabbit Monoclonal to Catalase
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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Zebrafish
Western blot analysis of Catalase on different lysates with Rabbit anti-Catalase antibody (ET1703-31) at 1/1,000 dilution.
Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si Catalase cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Catalase Recombinant Rabbit Monoclonal Antibody [JM11-12] (ET1703-31)
Immunogen
Synthetic peptide within human catalase aa 51-100.
Host
Rabbit
Positive Control
293T cell lysate, Hela cell lysate, HepG2 cell lysate, mouse liver tissue, rat liver tissue, human liver tissue, human uterus tissue, mouse prostate tissue, rat adrenal gland tissue., C2C12, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-12
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 60 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Catalase
SYNONYMS
Cas1 antibody;CAT antibody;CATA_HUMAN antibody;Catalase antibody;Cs1 antibody;MGC138422 antibody;MGC138424 antibody
SEQUENCE SIMILARITIES
Belongs to the catalase family.
POST-TRANSLATIONAL MODIFICATION
The N-terminus is blocked.
SUBCELLULAR LOCATION
Peroxisome.
FUNCTION
Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four iron-containing heme groups that allow the enzyme to react with hydrogen peroxide. The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. The pH optimum for other catalases varies between 4 and 11 depending on the species. The optimum temperature also varies by species.
CITATIONS
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Wei W, Zhang Q, Jin T, et al
Quantitative Proteomics Characterization of the Effect and Mechanism of Trichostatin A on the Hippocampus of Type II Diabetic Mice
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WB
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IHC
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Mouse
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Citation
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Sun, Y., Xu, H., Tan, B., Yi, Q., Liu, H., Chen, T., Xiang, H., Wang, R., Xie, Q., Tian, J., & Zhu, J.
Andrographolide protects bone marrow mesenchymal stem cells against glucose and serum deprivation under hypoxia via the NRF2 signaling pathway
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WB
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Rat
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Citation
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Sun, Y., He, Y., Tong, J., Liu, D., Zhang, H., He, T., & Bi, Y.
All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis
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WB
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Human
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Citation
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Andrographolide-treated bone marrow mesenchymal stem cells-derived conditioned medium protects cardiomyocytes from injury by metabolic remodeling
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WB
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Rat
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Citation
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