IKBKE Recombinant Rabbit Monoclonal Antibody [JU06-72] (ET1706-20)

UNIPROT #

Q14164

PROTEIN NAME

IKBKE

SYNONYMS

I kappa B kinase epsilon antibody;I-kappa-B kinase epsilon antibody;IkBKE antibody;IKK related kinase epsilon antibody;IKK-E antibody;IKK-epsilon antibody;IKK-i antibody;IKKE antibody;IKKE_HUMAN antibody;IKKepsilon antibody;IKKI antibody;Inducible I kappa B kinase antibody;Inducible I kappa-B kinase antibody;Inducible IkappaB kinase antibody;Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody;Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody;Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody;KIAA0151 antibody;MGC125294 antibody;MGC125295 antibody;MGC125297 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

TISSUE SPECIFICITY

Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated and phosphorylated by IKBKB/IKKB. Phosphorylation at Ser-172 is enhanced by the interaction with DDX3X. Phosphorylated at Thr-501 upon IFN activation.; Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.; 'Lys-63'-linked polyubiquitinated at Lys-30 and Lys-401 by TRAF2:BIRC2 and TRAF2:BIRC3 complexes. Ubiquitination is induced by LPS, TNFA and interleukin-1 and required for full kinase activity and KF-kappa-B pathway activation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKα and IKKβ. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IκBα on Ser 32 and Ser 36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.