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Phospho-Smad3 (S423/S425) Recombinant Rabbit Monoclonal Antibody [ST0493] (ET1609-41)

Price: Regular price $385.00

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Catalog# ET1609-41

Rabbit Monoclonal to Phospho-Smad3 (S423/S425)

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reacts with

  • Human

  • Mouse

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-Smad3 (S423/S425) Recombinant Rabbit Monoclonal Antibody [ST0493] (ET1609-41)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser423 and 425 of human smad3.

Specificity

Phospho-smad3 (s423/s425) (st0493) rabbit mab detects endogenous levels of smad3 only when dually phosphorylated at serines 423 and 425, and may detect smad2 phosphorylated at serines 465 and 467.

Host

Rabbit

Modification

Phospho

Modification Site

S423/S425

Positive Control

A549 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, C2C12 treated with 5ng/mL TGF-beta1 for 24 hours whole cell lysate, HeLa starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, NIH/3T3 starved overnight then treated with 10ng/mL TGF-β1 for 30 minutes cell lysate, A549, human large intestine tissue, mouse skin tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST0493

RRID

AB_3069851

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:500

  • IF-Tissue

  • 1:200

  • IHC-P

  • 1:200-1:500

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 48 kDa

Isotype

IgG

TARGET

UNIPROT #

P84022

PROTEIN NAME

Phospho-Smad3 (S423/S425)

SYNONYMS

DKFZP586N0721 antibody;DKFZp686J10186 antibody;hMAD 3 antibody;hMAD-3 antibody;hSMAD3 antibody;HSPC193 antibody;HST17436 antibody;JV15 2 antibody;JV15-2 antibody;JV152 antibody;LDS1C antibody;LDS3 antibody;MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody;MAD homolog 3 antibody;Mad homolog JV15 2 antibody;Mad protein homolog antibody;MAD, mothers against decapentaplegic homolog 3 antibody;Mad3 antibody;MADH 3 antibody;MADH3 antibody;MGC60396 antibody;Mothers against decapentaplegic homolog 3 antibody;Mothers against DPP homolog 3 antibody;SMA and MAD related protein 3 antibody;SMAD 3 antibody;SMAD antibody;SMAD family member 3 antibody;SMAD, mothers against DPP homolog 3 antibody;Smad3 antibody;SMAD3_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and threonine residues. Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment. Ser-208 is the main site of MAPK-mediated phosphorylation. CDK-mediated phosphorylation occurs in a cell-cycle dependent manner and inhibits both the transcriptional activity and antiproliferative functions of SMAD3. This phosphorylation is inhibited by flavopiridol. Maximum phosphorylation at the G(1)/S junction. Also phosphorylated on serine residues in the C-terminal SXS motif by TGFBR1 and ACVR1. TGFBR1-mediated phosphorylation at these C-terminal sites is required for interaction with SMAD4, nuclear location and transactivational activity, and appears to be a prerequisite for the TGF-beta mediated phosphorylation in the linker region. Dephosphorylated in the C-terminal SXS motif by PPM1A. This dephosphorylation disrupts the interaction with SMAD4, promotes nuclear export and terminates TGF-beta-mediated signaling. Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Phosphorylated by PDPK1.; Acetylation in the nucleus by EP300 in the MH2 domain regulates positively its transcriptional activity and is enhanced by TGF-beta.; Poly-ADP-ribosylated by PARP1 and PARP2. ADP-ribosylation negatively regulates SMAD3 transcriptional responses during the course of TGF-beta signaling.; Ubiquitinated. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD3 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD3 and regulating its turnover (By similarity). Undergoes STUB1-mediated ubiquitination and degradation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. Human Smad3 is subject to phosphorylation by TGFβ on specific amino acid residues, including Ser 208.

CITATIONS

  • FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors

  • Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.

    Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways

  • Jiang Shuanghong,et al

    ARHGAP4 promotes colon cancer metastasis through the TGF-β signaling pathway and may be associated with T cell exhaustion

  • Knockdown of Secernin 1 inhibit cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas

  • Hu, X., Chen, M., Cao, X., Yuan, X., Zhang, F., & Ding, W.

    TGF-β-Containing Small Extracellular Vesicles From PM2.5-Activated Macrophages Induces Cardiotoxicity

  • Xu, L., Tan, B., Huang, D., Yuan, M., Li, T., Wu, M., & Ye, C.

    Remdesivir Inhibits Tubulointerstitial Fibrosis in Obstructed Kidneys. Frontiers in pharmacology, 12, 626510.

  • Yanfang Bai,et al

    Tanshinone I improves renal fibrosis by promoting gluconeogenesis through upregulation of peroxisome proliferator-activated receptor-γ coactivator 1α

  • Fibulin-3 regulates the inhibitory effect of TNF-α on chondrocyte differentiation partially via the TGF-β/Smad3 signaling pathway

  • Wu Bibo,et al

    PD-1 Inhibitor Aggravate Irradiation-Induced Myocardial Fibrosis by Regulating TGF-β1/Smads Signaling Pathway via GSDMD-Mediated Pyroptosis

  • Chloroquine Intervenes Nephrotoxicity of Nilotinib through Deubiquitinase USP13‐Mediated Stabilization of Bcl‐XL

  • Liao Taiyang,et al

    Suppression of NUPR1 in fibroblast-like synoviocytes reduces synovial fibrosis via the Smad3 pathway

  • Treatment with a PPAR-γ Agonist Protects Against Hyperuricemic Nephropathy in a Rat Model

  • Bai Yuanmei,et al

    Experimental study on H2O2 activation of HSC-T6 and hepatic fibrosis in cholestatic mice by "Yajieshaba"

  • TBX20 Contributes to Balancing the Differentiation of Perivascular Adipose-Derived Stem Cells to Vascular Lineages and Neointimal Hyperplasia

  • Wang, Y., Wu, M., Chen, D., Tan, B., Lin, P., Huang, D., & Ye, C.

    SDMA attenuates renal tubulointerstitial fibrosis through inhibition of STAT4

  • Li, Y., Guo, F., Huang, R., Ma, L., & Fu, P.

    Natural flavonoid pectolinarigenin alleviated kidney fibrosis via inhibiting the activation of TGFβ/SMAD3 and JAK2/STAT3 signaling. International immunopharmacology, 91, 107279.

  • Enping Li,et al

    ALDH2 attenuates radiation-induced lung injury by inhibiting ROS and epithelial-mesenchymal transition mediated by the TGF-β1/Smad pathway

  • Niclosamide-loaded nanoparticles (Ncl-NPs) reverse pulmonary fibrosis in vivo and in vitro

  • Wu, M., Yang, F., Huang, D., & Ye, C.

    Tanshinone I attenuates fibrosis in fibrotic kidneys through down-regulation of inhibin beta-A

  • Dandan Wu,et al

    RRM2 Regulates Hepatocellular Carcinoma Progression Through Activation of TGF-β/Smad Signaling and Hepatitis B Virus Transcription

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