TRPV6 Rabbit Polyclonal Antibody (HA500333)
Catalog# HA500333
Rabbit Polyclonal to TRPV6
Tested for
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WB
-
IHC-P
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IF-Cell
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FC
Reacts with
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Human
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Mouse
-
Rat
Western blot analysis of TRPV6 on different lysates with Rabbit anti-TRPV6 antibody (HA500333) at 1/500 dilution.
Lane 1: SW480 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6)
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500333) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: SW480 cell lysate
Lane 2: SK-Br-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 42 kDa (unglycosylated monomeric form of TRPV6)
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500333) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.