Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] (ET1701-80)
Catalog# ET1701-80
Rabbit Monoclonal to Actin
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: PC-12 cell lysate (15 µg/Lane)
Lane 5: Mouse brain tissue lysate (20 µg/Lane)
Lane 6: Mouse colon tissue lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (20 µg/Lane)
Lane 8: Rat colon tissue lysate (20 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Safety datasheet
Select your chosen country/region
- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1701-80_Europe.pdf
- No MSDS Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] (ET1701-80)
Immunogen
Synthetic peptide within human actin aa 45-80 / 377.
Host
Rabbit
Positive Control
HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse colon tissue lysate, rat brain tissue lysate, rat colon tissue lysate, human colon carcinoma tissue, hybrid fish (crucian-carp) brain tissue lysate, hybrid fish (crucian-carp) kidney tissue lysate, NIH/3T3, mouse cardiac muscle tissue, mouse smooth muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ09-29
RRID
APPLICATION DILUTION
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WB
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1:10,000-1:50,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:1,000
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IHC-P
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Actin
SYNONYMS
a actin antibody;ACTA antibody;ACTA1 antibody;Actin alpha skeletal muscle antibody;Actin antibody;actin, alpha 1, skeletal muscle 1 antibody;actin, alpha 1, skeletal muscle antibody;Actin, alpha skeletal muscle antibody;actina antibody;actine antibody;ACTS_HUMAN antibody;aktin antibody;Alpha Actin 1 antibody;Alpha skeletal muscle Actin antibody;alpha skeletal muscle antibody;alpha-actin antibody;Alpha-actin-1 antibody;ASMA antibody;CFTD antibody;CFTD1 antibody;CFTDM antibody;MPFD antibody;NEM1 antibody;NEM2 antibody;NEM3 antibody;nemaline myopathy type 3 antibody
SEQUENCE SIMILARITIES
Belongs to the actin family.
POST-TRANSLATIONAL MODIFICATION
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.; Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-75 by SETD3.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
SUBCELLULAR LOCATION
Cytoplasm, Cytoskeleton.
FUNCTION
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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