VEGF Recombinant Rabbit Monoclonal Antibody [SP07-01] (ET1604-28)
Catalog# ET1604-28
Rabbit Monoclonal to VEGF
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VEGF antibody (ET1604-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Safety datasheet
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- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1604-28_Europe.pdf
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Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
VEGF Recombinant Rabbit Monoclonal Antibody [SP07-01] (ET1604-28)
Immunogen
Synthetic peptide within human vegf aa 183-232 / 232.
Host
Rabbit
Positive Control
Hela, MCF-7, SHG-44, SH-SY5Y, human kidney tissue, rat eyeball tissue, mouse cerebellum tissue, Hela, mouse hippocampus tissue, mouse brain tissue, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP07-01
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 27 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
VEGF
SYNONYMS
Folliculostellate cell-derived growth factor antibody;Glioma-derived endothelial cell mitogen antibody;MGC70609 antibody;MVCD1 antibody;Vascular endothelial growth factor A antibody;vascular endothelial growth factor A121 antibody;vascular endothelial growth factor A165 antibody;vascular endothelial growth factor antibody;Vascular permeability factor antibody;VEGF A antibody;Vegf antibody;VEGF-A antibody;VEGF120 antibody;Vegfa antibody;VEGFA_HUMAN antibody;VPF antibody
SEQUENCE SIMILARITIES
Belongs to the PDGF/VEGF growth factor family.
TISSUE SPECIFICITY
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
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