MELK Recombinant Rabbit Monoclonal Antibody [PSH03-55] (HA722014)
Catalog# HA722014
Rabbit Monoclonal to MELK
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MELK on different lysates with Rabbit anti-MELK antibody (HA722014) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 72 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 72 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.