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SGK1 Recombinant Rabbit Monoclonal Antibody [SC05-71] (ET1610-19)

Price: Regular price $385.00

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Catalog# ET1610-19

Rabbit Monoclonal to SGK1

Tested for

  • WB

  • IHC-P

  • IP

  • FC

  • IF-Tissue

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

SGK1 Recombinant Rabbit Monoclonal Antibody [SC05-71] (ET1610-19)

Immunogen

Synthetic peptide within human sgk1 aa 40-80.

Host

Rabbit

Positive Control

Mouse kidney tissue lysates, human kidney tissue lysates, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, mouse liver tissue, 293.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC05-71

RRID

AB_3069902

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P:1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

  • IF-Tissue

  • 1:200

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 49 kDa

Isotype

IgG

TARGET

UNIPROT #

O00141

PROTEIN NAME

SGK1

SYNONYMS

OTTHUMP00000017247 antibody;Serine/threonine protein kinase SGK antibody;Serine/threonine protein kinase Sgk1 antibody;Serine/threonine-protein kinase Sgk1 antibody;Serum and glucocorticoid regulated kinase antibody;Serum/glucocorticoid regulated kinase 1 antibody;Serum/glucocorticoid regulated kinase antibody;Serum/glucocorticoid-regulated kinase 1 antibody;SGK 1 antibody;SGK antibody;SGK1 antibody;Sgk1 variant i3 antibody;SGK1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Regulated by phosphorylation. Activated by phosphorylation on Ser-422 by mTORC2, transforming it into a substrate for PDPK1 which phosphorylates it on Thr-256. Phosphorylation on Ser-397 and Ser-401 are also essential for its activity. Phosphorylation on Ser-78 by MAPK7 is required for growth factor-induced cell cycle progression.; Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane, Mitochondrion.

FUNCTION

Serum- and glucocorticoid-regulated kinase (SGK), also known as SGK1, is a serine/threonine protein kinase and a member of the "AGC" subfamily, which includes protein kinases A, G, and C. SGK plays an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK contains a catalytic domain, which is most similar to Akt1 (also known as protein kinase B or PKB). SGK is a downstream target of PI 3-kinase-stimulated growth factor signaling, with 3-phosphoinositide-dependent protein kinase 1 (PDK1) capable of phosphorylating the activation-loop of SGK at Threonine-256. The adrenal corticosteroid hormone, Aldosterone, induces the transcription of SGK, which mediates Na + transport by stimulating epithelial sodium channel activity. The SGK promoter contains a glucocorticoid response element and an SP-1 regulatory element, and is a transcriptional target for p53. SGK is also a component of the p38 MAPK-mediated response to hyperosmotic stress. The human SGK gene maps to chromosome 6q23 and encodes the 431-amino acid SGK protein.

CITATIONS

  • Su, M., Zhan, L., Zhang, Y., & Zhang, J.

    Yes-activated protein promotes primary resistance of BRAF V600E mutant metastatic colorectal cancer cells to mitogen-activated protein kinase pathway inhibitors

  • Ma Yu,et al

    Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction

  • Li, S., Pei, H., Huang, Y., Liu, D., Yang, L., Zhang, Q., & Wang, Z.

    Experimental study on the effect of chlorhexidine gluconate (CG)-induced atrial fibrillation on renal water and sodium metabolism

  • Glucocorticoids ameliorate cardiorenal syndrome through the NPR1/SGK1 pathway in natriuretic peptide receptor A‑heterozygous mice

  • He, J., Lin, M., Zhang, X., Zhang, R., Tian, T., Zhou, Y., Dong, W., Yang, Y., Sun, X., Dai, Y., Xu, Y., Zhang, Z., Xu, M., Lei, Q. Y., Xu, Y., & Lv, L.

    TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential

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