CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63] (HA721321)

UNIPROT #

P08571

PROTEIN NAME

CD14

SYNONYMS

CD 14 antibody;CD_antigen=CD14 antibody;CD14 antibody;CD14 antigen antibody;CD14 molecule antibody;CD14_HUMAN antibody;LPS-R antibody;Mo2 antibody;Monocyte differentiation antigen CD14 antibody;Monocyte differentiation antigen CD14 urinary form antibody;Monocyte differentiation antigen CD14, membrane-bound form antibody;Myeloid cell specific leucine rich glycoprotein antibody;Myeloid cell-specific leucine-rich glycoprotein antibody

TISSUE SPECIFICITY

Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

POST-TRANSLATIONAL MODIFICATION

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

SUBCELLULAR LOCATION

Cell membrane, Secreted, Golgi apparatus, Membrane raft.

FUNCTION

CD14 is a 55-kDa protein found as a glycosylphosphatidylinositol (GPI)- anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, and as a soluble protein in the blood. Its main function is to serve as a receptor for lipopolysaccharide (LPS). CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14. Besides its role in endotoxin signaling, it has been proposed that CD14 is involved in the transportation of other lipids, cell-cell interactions during different immune responses, and recognition of apoptotic cells. CD14 is highly expressed on the surface of monocytes/macrophages and strongly up-regulated during the differentiation of monocytic precursor cells into mature monocytes. Therefore, CD14 has been commonly used as a differentiation marker for monocytes/macrophages. An antibody to CD14 also labels Langerhans' cells and dendritic cells.