Fragilis Recombinant Rabbit Monoclonal Antibody [JU73-02] (ET1706-09)
Catalog# ET1706-09
Rabbit Monoclonal to Fragilis
Tested for
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Fragilis on different lysates with Rabbit anti-Fragilis antibody (ET1706-09) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: THP-1 cell lysate
Lane 4: 293T cell lysate (negative)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: THP-1 cell lysate
Lane 4: 293T cell lysate (negative)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
