Oct4 Mouse Monoclonal Antibody [A3-C11] (EM100306)

UNIPROT #

Q01860

PROTEIN NAME

Oct4

SYNONYMS

Octamer binding transcription factor 4 antibody;MGC22487 antibody;Oct 3 antibody;Oct 4 antibody;Oct-3 antibody;Oct-4 antibody;OCT3 antibody;Oct4 antibody;Octamer binding protein 3 antibody;Octamer binding protein 4 antibody;Octamer binding transcription factor 3 antibody;Octamer-binding protein 3 antibody;Octamer-binding protein 4 antibody;Octamer-binding transcription factor 3 antibody;OTF 3 antibody;OTF 4 antibody;OTF-3 antibody;OTF3 antibody;OTF4 antibody;PO5F1_HUMAN antibody;POU class 5 homeobox 1 antibody;POU domain class 5 transcription factor 1 antibody;POU domain transcription factor OCT4 antibody;POU domain, class 5, transcription factor 1 antibody;POU-type homeodomain-containing DNA-binding protein antibody;POU5F1 antibody

SEQUENCE SIMILARITIES

Belongs to the POU transcription factor family. Class-5 subfamily.

TISSUE SPECIFICITY

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

DEVELOPMENTAL STAGE

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

POST-TRANSLATIONAL MODIFICATION

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.; Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.