CD40 Rabbit Polyclonal Antibody (ER1803-54)
Catalog# ER1803-54
Rabbit Polyclonal to CD40
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Rat
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Mouse
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of CD40 on Daudi cell lysates with Rabbit anti-CD40 antibody (ER1803-54) at 1/2,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-54) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-54) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
