CD80 Mouse Monoclonal Antibody [8-E5] (M1007-10)
Catalog# M1007-10
Mouse Monoclonal to CD80/B7-1
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WB
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IHC-P
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FC
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Human
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Mouse
Lysates/proteins at 40 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
CD80 Mouse Monoclonal Antibody [8-E5] (M1007-10)
Immunogen
Synthetic peptide within human cd80 aa 74-118 / 288.
Host
Mouse
Positive Control
Human brain tissue lysates, human brain tissue, Raji.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
8-E5
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
CD80/B7-1
SYNONYMS
Activation B7-1 antigen antibody;B lymphocyte activation antigen B7 antibody;B7 antibody;B7-1 antibody;B7-1 antigen antibody;B7.1 antibody;BB1 antibody;CD28 antigen ligand 1 antibody;CD28LG antibody;CD28LG1 antibody;CD80 antibody;CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody;CD80 antigen antibody;CD80 molecule antibody;CD80_HUMAN antibody;Costimulatory factor CD80 antibody;costimulatory molecule variant IgV-CD80 antibody;CTLA-4 counter-receptor B7.1 antibody;LAB7 antibody;T-lymphocyte activation antigen CD80 antibody
TISSUE SPECIFICITY
Expressed on activated B-cells, macrophages and dendritic cells.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
CD80/B7-1 is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines. B7-1 is a costimulatory molecule for the activation of both CD4+ and CD8+ T lymphocytes that prevents the induction of clonal anergy. Thus, the transfer of B7-1 genes into tumor cells can induce protective immunity and lead to tumor rejection of some tumors in model systems of in vivo tumor growth.
CITATIONS
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Han, Z., Ma, J., Han, Y., Yuan, G., Jiao, R., & Meng, A.
Irisin attenuates acute lung injury by suppressing the pyroptosis of alveolar macrophages
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IF
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Mouse
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Citation
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Jinghai Chen & Da-Zhi Wang
Therapeutic role of miR-19a/19b in cardiac regeneration and protection from myocardial infarction
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IHC
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Mouse
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Citation
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Xia, H., Wang, Y., Dai, J., Zhang, X., Zhou, J., Zeng, Z., & Jia, Y.
Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells
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WB
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Mouse
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Citation
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Liang, L., Xu, W., Shen, A., Fu, X., Cen, H., Wang, S., Lin, Z., Zhang, L., Lin, F., Zhang, X., Zhou, N., Chang, J., Chen, Z. S., Li, C., & Yu, X.
Inhibition of YAP1 activity ameliorates acute lung injury through promotion of M2 macrophage polarization
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WB
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Mouse
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Citation
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