LEF1 Recombinant Rabbit Monoclonal Antibody [JE52-28] (HA721992)
Catalog# HA721992
Rabbit Monoclonal to LEF1
Tested for
-
WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (HA721992) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
Lane 4: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 35-55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
Lane 4: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 35-55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721992) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.