CD10 Rabbit Polyclonal Antibody (0805-4)
Catalog# 0805-4
Rabbit Polyclonal to CD10
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (0805-4) at 1/2,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: Ramos cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: LNCaP cell lysate
Lane 2: Ramos cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.