Jagged1 Recombinant Rabbit Monoclonal Antibody [JF96-06] (ET1702-63)
Catalog# ET1702-63
Rabbit Monoclonal to Jagged1
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Jagged1 on different lysates with Rabbit anti-Jagged1 antibody (ET1702-63) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: bEND.3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: bEND.3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.