Wnt3a Rabbit Polyclonal Antibody (HA500193)
Catalog# HA500193
Rabbit Polyclonal to Wnt3a
Tested for
-
WB
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IF-Cell
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IHC-P
Reacts with
-
Human
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Wnt3a on different lysates with Rabbit anti-Wnt3a antibody (HA500193) at 1/1,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-Wnt3a cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500193) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-Wnt3a cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 36 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500193) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
