ID2 Mouse Monoclonal Antibody [A4-D4] (M1301-2)
Catalog# M1301-2
Mouse Monoclonal to ID2
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WB
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IHC-P
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Human
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Mouse
Lane 1: HEK-293 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: SK-OV-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1301-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
ID2 Mouse Monoclonal Antibody [A4-D4] (M1301-2)
Immunogen
Recombinant protein with human id2 aa 1-80 / 134.
Host
Mouse
Positive Control
HEK-293 cell lysate, HT-29 cell lysate, SK-OV-3 cell lysate, human pancreatic cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A4-D4
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:10,000
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IHC-P
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 15 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
ID2
SYNONYMS
bHLHb26 antibody;Cell growth inhibiting gene 8 antibody;class B basic helix loop helix protein 26 antibody;Class B basic helix-loop-helix protein 26 antibody;DNA binding protein inhibitor ID 2 antibody;DNA binding protein inhibitor ID2 antibody;DNA-binding protein inhibitor ID-2 antibody;GIG 8 antibody;GIG8 antibody;Helix loop helix protein ID2 antibody;ID2 antibody;ID2_HUMAN antibody;ID2A antibody;ID2H antibody;Inhibitor of differentiation 2 antibody;Inhibitor of DNA binding 2 antibody;Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody;MGC26389 antibody
TISSUE SPECIFICITY
Highly expressed in early fetal tissues, including those of the central nervous system.
DEVELOPMENTAL STAGE
Found in most early fetal tissues but not in the corresponding mature tissues.
SUBCELLULAR LOCATION
Cytoplasm, nucleus.
FUNCTION
DNA-binding protein inhibitor ID-2, is an inhibitor of differentiation protein, which, along with ID1, ID3, and ID4, plays a key role in the regulation of lineage commitment, cell fate decisions, and in the timing of differentiation during neurogenesis, lymphopoiesis, and neovascularization (angiogenesis). ID-2 may be an inhibitor of tissue-specific gene expression.
CITATIONS
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Heo, J., Lee, J., Nam, Y. J., Kim, Y., Yun, H., Lee, S., Ju, H., Ryu, C. M., Jeong, S. M., Lee, J., Lim, J., Cho, Y. M., Jeong, E. M., Hong, B., Son, J., & Shin, D. M.
The CDK1/TFCP2L1/ID2 cascade offers a novel combination therapy strategy in a preclinical model of bladder cancer
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IF
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Human
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Citation
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