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6X His tag Mouse Monoclonal Antibody [8-F0] (M0812-3)

Price: Regular price $350.00

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Catalog# M0812-3

Mouse Monoclonal to 6X His tag

Tested for

  • WB

  • IP

Reacts with

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Mouse monoclonal primary

Product Name

6X His tag Mouse Monoclonal Antibody [8-F0] (M0812-3)

Immunogen

Synthetic peptide immune sequence is chhhhhhhh.

Host

Mouse

Positive Control

C-terminal His-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8-F0

RRID

AB_3072999

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IP

  • 2-5 µg/ml.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.

Concentration

2ug/ul

Purification

Protein G affinity purified.

Isotype

IgG2a

TARGET

PROTEIN NAME

6X His tag

SYNONYMS

6 His epitope tag antibody;Hexa His tag antibody;HHHHHH epitope tag antibody;HHHHHH tag antibody;His tag antibody;Polyhistidine Tag antibody

FUNCTION

A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.

CITATIONS

  • Ronggui Hu

    Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK

  • Zhaoyun Zhang

    High glucose-induced ubiquitination of G6PD leads to the injury of podocytes.

  • Zhao, J., Xu, J., Yang, T., Yu, X., Cheng, C., Zhang, T., Ren, Z., Li, N., Yang, F., & Li, G.

    Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody

  • CYNTHIA JU,Guiying Li

    Interaction of AIM with insulin-like growth factor-binding protein-4

  • Yang, H., Dong, Y., Bian, Y., Xu, N., Wu, Y., Yang, F., Du, Y., Qin, T., Chen, S., Peng, D., & Liu, X.

    The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling

  • Yahui Li ,et al

    Coronavirus M protein promotes mitophagy over virophagy by recruiting PDPK1 to phosphorylate SQSTM1 at T138

  • Yongzhong Liu

    POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation

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