6X His tag Mouse Monoclonal Antibody [8-F0] (M0812-3)
Catalog# M0812-3
Mouse Monoclonal to 6X His tag
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WB
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IP
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Species independent
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein
Specifications
Product Type
Mouse monoclonal primary
Product Name
6X His tag Mouse Monoclonal Antibody [8-F0] (M0812-3)
Immunogen
Synthetic peptide immune sequence is chhhhhhhh.
Host
Mouse
Positive Control
C-terminal His-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
8-F0
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IP
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2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Isotype
IgG2a
TARGET
PROTEIN NAME
6X His tag
SYNONYMS
6 His epitope tag antibody;Hexa His tag antibody;HHHHHH epitope tag antibody;HHHHHH tag antibody;His tag antibody;Polyhistidine Tag antibody
FUNCTION
A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.
CITATIONS
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Ronggui Hu
Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK
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Co-IP
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IF
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Escherichia coli
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Citation
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Zhaoyun Zhang
High glucose-induced ubiquitination of G6PD leads to the injury of podocytes.
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WB
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Mouse
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Citation
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Zhao, J., Xu, J., Yang, T., Yu, X., Cheng, C., Zhang, T., Ren, Z., Li, N., Yang, F., & Li, G.
Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody
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WB
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Escherichia coli
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Citation
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CYNTHIA JU,Guiying Li
Interaction of AIM with insulin-like growth factor-binding protein-4
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Co-IP
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WB
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Human
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Citation
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Yang, H., Dong, Y., Bian, Y., Xu, N., Wu, Y., Yang, F., Du, Y., Qin, T., Chen, S., Peng, D., & Liu, X.
The influenza virus PB2 protein evades antiviral innate immunity by inhibiting JAK1/STAT signalling
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WB
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Human
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Citation
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Yahui Li ,et al
Coronavirus M protein promotes mitophagy over virophagy by recruiting PDPK1 to phosphorylate SQSTM1 at T138
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WB
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mice
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Citation
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Yongzhong Liu
POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation
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WB
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Human
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Citation
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