HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]
Catalog# M1005-3
HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]
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WB
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ELISA
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Human
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HRP
Overview
Product Name
HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified Human IgG heavy chain.
Species Reactivity
Human
Validated Applications
WB, ELISA
Molecular Weight
Predicted band size: 50 kDa
Positive Control
Human serum lysates.
Conjugation
HRP
Clone Number
54-76
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Isotype
IgG2a
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
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ELISA
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1:5,000
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Background References
暂无
Subcellular Location
serum plasma
Images
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Western blot analysis of Mouse anti-Human IgG heavy chain on human serum lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1005-3, 1/1000) was used in 5% BSA at room temperature for 2 hours.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"