ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11] (ET1703-60)
Catalog# ET1703-60
Rabbit Monoclonal to ADAM10
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Human lung tissue lysate (30 µg/Lane)
Lane 4: RAW264.7 cell lysate (15 µg/Lane)
Lane 5: Mouse placenta tissue lysate (30 µg/Lane)
Lane 6: Mouse brain tissue lysate (30 µg/Lane)
Lane 7: Rat brain tissue lysate (30 µg/Lane)
Lane 8: Rat spleen tissue lysate (30 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75/100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-60) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11] (ET1703-60)
Immunogen
Synthetic peptide within human adam10 aa 715-748 / 748.
Host
Rabbit
Positive Control
Jurkat cell lysate, HeLa cell lysate, human lung tissue lysate, RAW264.7 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat spleen tissue lysate, human thyroid carcinoma tissue, human stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM32-11
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 84 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ADAM10
SYNONYMS
A disintegrin and metalloprotease domain 10 antibody;A disintegrin and metalloproteinase domain 10 antibody;AD 10 antibody;AD10 antibody;AD18 antibody;ADA10_HUMAN antibody;ADAM 10 antibody;ADAM metallopeptidase domain 10 antibody;ADAM10 antibody;CD 156c antibody;CD156c antibody;CD156c antigen antibody;CDw156 antibody;disintegrin and metalloproteinase domain containing protein 10 antibody;Disintegrin and metalloproteinase domain-containing protein 10 antibody;HsT 18717 antibody;HsT18717 antibody;Kuz antibody;Kuzbanian antibody;Kuzbanian protein homolog antibody;Kuzbanian, Drosophila, homolog of antibody;MADM antibody;Mammalian disintegrin metalloprotease antibody;Mammalian disintegrin-metalloprotease antibody;RAK antibody
TISSUE SPECIFICITY
Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
POST-TRANSLATIONAL MODIFICATION
The precursor is cleaved by furin and PCSK7.
SUBCELLULAR LOCATION
Cell membrane. Endomembrane system.
FUNCTION
ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand) a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM10 is a TNF-processing enzyme that cleaves pro-TNF, a membrane-bound precusor protein, at Ala 76-Val 77, which causes membrane shedding of soluble TNF.
CITATIONS
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Yuzhen Huang,et al
ADAM10-mediated β-klotho degradation: a key to FGF21 resistance in senescent vascular endothelial cells
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Co-IP
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Mouse
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Citation
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