AIF Recombinant Rabbit Monoclonal Antibody [SZ05-01] (ET1603-4)

UNIPROT #

O95831

PROTEIN NAME

AIF

SYNONYMS

AIFM1 antibody;AIFM1_HUMAN antibody;Apoptosis inducing factor 1, mitochondrial antibody;Apoptosis inducing factor antibody;Apoptosis inducing factor, mitochondrion associated, 1 antibody;Apoptosis-inducing factor 1 antibody;CMTX4 antibody;COWCK antibody;COXPD6 antibody;Harlequin antibody;Hq antibody;mAIF antibody;MGC111425 antibody;MGC5706 antibody;mitochondrial antibody;Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody;neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody;PDCD 8 antibody;PDCD8 antibody;Programmed cell death 8 (apoptosis inducing factor) antibody;Programmed cell death 8 antibody;Programmed cell death 8 isoform 1 antibody;Programmed cell death 8 isoform 2 antibody;Programmed cell death 8 isoform 3 antibody;Programmed cell death protein 8 antibody;Programmed cell death protein 8 mitochondrial antibody;Programmed cell death protein 8 mitochondrial precursor antibody;Programmed cell death protein 8 mitochondrial precursor antibody;Striatal apoptosis inducing factor antibody

SEQUENCE SIMILARITIES

Belongs to the FAD-dependent oxidoreductase family.

TISSUE SPECIFICITY

Expressed in all tested tissues. Detected in muscle and skin fibroblasts (at protein level).; [Isoform 3]: Brain specific.; [Isoform 4]: Expressed in all tested tissues except brain.; [Isoform 5]: Isoform 5 is frequently down-regulated in human cancers.

POST-TRANSLATIONAL MODIFICATION

Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.; Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.

SUBCELLULAR LOCATION

Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus, Membrane.

FUNCTION

A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.