ALDH1A1 Mouse Monoclonal Antibody [8F1] (EM1801-07)
Catalog# EM1801-07
Mouse Monoclonal to ALDH1A1
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
IHC-Fr
Reacts with
-
Human
-
Rat
-
Mouse
Knock-Out or Knock-Down validated
Western blot analysis of ALDH1A1 on different lysates with Mouse anti-ALDH1A1 antibody (EM1801-07) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HT-29 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.