Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02] (ET1611-28)
Catalog# ET1611-28
Rabbit Monoclonal to Angiopoietin 1
Tested for
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WB
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Angiopoietin 1 on different lysates with Rabbit anti-Angiopoietin 1 antibody (ET1611-28) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD Angiopoietin 1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 65 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD Angiopoietin 1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 65 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
