ASC1 Rabbit Polyclonal Antibody (HA500460)
Catalog# HA500460
Rabbit Polyclonal to ASC1
Tested for
-
WB
Reacts with
-
Human
-
Rat
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA500460) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87MG cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87MG cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500460) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.