ATG12 Mouse Monoclonal Antibody [1-11] (M1701-4)
Catalog# M1701-4
Mouse Monoclonal to ATG12
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Specifications
Product Type
Mouse monoclonal primary
Product Name
ATG12 Mouse Monoclonal Antibody [1-11] (M1701-4)
Immunogen
Synthetic peptide within human atg12 91-140 / 140.
Host
Mouse
Positive Control
THP-1, MCF-7. PC-3M, SH-SY5Y, human liver cancer tissue, human stomach cancer tissue, human fetal skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1-11
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 15 kDa
Isotype
IgE
TARGET
UNIPROT #
PROTEIN NAME
ATG12
SYNONYMS
APG12-like antibody;APG12L antibody;ATG12 antibody;ATG12 autophagy related 12 homolog (S. cerevisiae) antibody;ATG12 autophagy related 12 homolog antibody;ATG12_HUMAN antibody;Autophagy 12 antibody;Autophagy-related protein 12 antibody;FBR93 antibody;HAPG12 antibody;Ubiquitin-like protein ATG12 antibody
SEQUENCE SIMILARITIES
Belongs to the ATG12 family.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
Acetylated by EP300.
SUBCELLULAR LOCATION
Cytoplasm. Membrane.
FUNCTION
Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.
CITATIONS
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Weiqi Yan
Water Extract of Sporoderm-Broken Spores of Ganoderma lucidum Induces Osteosarcoma Apoptosis and Restricts Autophagic Flux
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WB
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IHC
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Mouse
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Citation
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