ATM Recombinant Rabbit Monoclonal Antibody [SI70-01] (ET1606-20)
Catalog# ET1606-20
Rabbit Monoclonal to ATM
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Rat
Lane 1: A549 cell lysate
Lane 2: CRC cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 351 kDa
Observed band size: 351 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ATM Recombinant Rabbit Monoclonal Antibody [SI70-01] (ET1606-20)
Immunogen
Synthetic peptide within human atm aa 1,951-2,000 / 3,056.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, NCCIT cell lysate, HEK-293 cell lysate, CRC cell lysate, HeLa, human testis tissue, human tonsil tissue, rat colon tissue, human pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI70-01
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 351 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ATM
SYNONYMS
A-T mutated antibody;A-T mutated homolog antibody;AT mutated antibody;AT1 antibody;ATA antibody;Ataxia telangiectasia mutated antibody;Ataxia telangiectasia mutated gene antibody;Ataxia telangiectasia mutated homolog (human) antibody;Ataxia telangiectasia mutated homolog antibody;ATC antibody;ATD antibody;ATDC antibody;ATE antibody;ATM antibody;ATM serine/threonine kinase antibody;ATM_HUMAN antibody;DKFZp781A0353 antibody;MGC74674 antibody;OTTHUMP00000232981 antibody;Serine protein kinase ATM antibody;Serine-protein kinase ATM antibody;Serine/threonine-protein kinase ATM antibody;Tefu antibody;TEL1 antibody;TEL1, telomere maintenance 1, homolog antibody;TELO1 antibody;Telomere fusion protein antibody
SEQUENCE SIMILARITIES
Belongs to the PI3/PI4-kinase family. ATM subfamily.
TISSUE SPECIFICITY
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. During the late stages of DNA damage response, dephosphorylated following deacetylation by SIRT7, leading to ATM deactivation.; Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.
SUBCELLULAR LOCATION
Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome By Similarity.
FUNCTION
The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiec-tases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction.
CITATIONS
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Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer
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WB
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human
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Citation
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Xia, Z., Li, M., Hu, M., Lin, Y., Atteh, L. L., Fu, W., Gao, L., Bai, M., Huang, C., Yue, P., Liu, Y., & Meng, W.
Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway
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WB
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Human
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Citation
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A novel and potent dihydroorotate dehydrogenase inhibitor suppresses the proliferation of colorectal cancer by inducing mitochondrial dysfunction and DNA damage
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WB
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Human
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Citation
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