B7H4 Recombinant Rabbit Monoclonal Antibody [PSH03-65] (HA722024)
Catalog# HA722024
Rabbit Monoclonal to B7H4
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of B7H4 on different lysates with Rabbit anti-B7H4 antibody (HA722024) at 1/1,000 dilution.
Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane)
Lane 2: HeLa cell lysate (negative) (30 µg/Lane)
Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane)
Lane 4: Mouse uterus tissue lysate (30 µg/Lane)
Lane 5: Rat ovary tissue lysate (15 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 31 kDa
Observed band size: 60-75 kDa
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane)
Lane 2: HeLa cell lysate (negative) (30 µg/Lane)
Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane)
Lane 4: Mouse uterus tissue lysate (30 µg/Lane)
Lane 5: Rat ovary tissue lysate (15 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 31 kDa
Observed band size: 60-75 kDa
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722024) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.