Bax Recombinant Rabbit Monoclonal Antibody [SZ3-07] (ET1603-34)
Catalog# ET1603-34
Rabbit Monoclonal to Bax
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Goat
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: bEnd.3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-34) at 1/20,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Bax Recombinant Rabbit Monoclonal Antibody [SZ3-07] (ET1603-34)
Immunogen
Synthetic peptide within human bax aa 1-50 / 192.
Host
Rabbit
Positive Control
HeLa cell lysate, MCF7 cell lysate, HEK-293 cell lysate, bEnd.3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, C2C12, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ3-07
RRID
APPLICATION DILUTION
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WB
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1:20,000
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IF-Cell
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1:100
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Bax
SYNONYMS
Apoptosis regulator BAX antibody;BAX antibody;Bax-protein antibody;BAX_HUMAN antibody;BAXA antibody;Baxdelta2G9 antibody;Baxdelta2G9omega antibody;Baxdelta2omega antibody;Bcl-2-like protein 4 antibody;BCL2 associated X protein antibody;BCL2 associated X protein omega antibody;BCL2 associated X protein transcript variant delta2 antibody;Bcl2-L-4 antibody;BCL2L4 antibody;membrane isoform alpha antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
SUBCELLULAR LOCATION
Mitochondrion membrane, Cytoplasm.
FUNCTION
In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. BAX is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence also suggests that activated BAX and/or Bak form an oligomeric pore, MAC in the MOM (mitochondrial outer membrane). This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases. This defines a direct role for BAX in mitochondrial outer membrane permeabilization. BAX activation is stimulated by various abiotic factors, including heat, hydrogen peroxide, low or high pH, and mitochondrial membrane remodeling. In addition, it can become activated by binding BCL-2, as well as non-BCL-2 proteins such as p53 and Bif-1. Conversely, BAX can become inactivated by interacting with VDAC2, Pin1, and IBRDC2.
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