Bcl-2 Mouse Monoclonal Antibody [9F1] (EM1701-82)
Catalog# EM1701-82
Mouse Monoclonal to Bcl-2
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WB
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IHC-P
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Human
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-82) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Bcl-2 Mouse Monoclonal Antibody [9F1] (EM1701-82)
Immunogen
Synthetic peptide within human bcl2 35-60 aa.
Host
Mouse
Positive Control
THP-1 cell lysates, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
9F1
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Bcl-2
SYNONYMS
Apoptosis regulator Bcl 2 antibody;Apoptosis regulator Bcl-2 antibody;Apoptosis regulator Bcl2 antibody;AW986256 antibody;B cell CLL/lymphoma 2 antibody;B cell leukemia/lymphoma 2 antibody;Bcl-2 antibody;Bcl2 antibody;BCL2_HUMAN antibody;C430015F12Rik antibody;D630044D05Rik antibody;D830018M01Rik antibody;Leukemia/lymphoma, B-cell, 2 antibody;Oncogene B-cell leukemia 2 antibody;PPP1R50 antibody;Protein phosphatase 1, regulatory subunit 50 antibody;Bcl 2 antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Expressed in a variety of tissues.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
SUBCELLULAR LOCATION
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
FUNCTION
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
CITATIONS
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Fang, T., Xu, R., Sun, S., He, Y., Yan, Y., Fu, H., Luo, H., Cao, Y., & Tao, M.
Caizhixuan hair tonic regulates both apoptosis and the PI3K/Akt pathway to treat androgenetic alopecia
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WB
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Mouse
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Citation
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Fan Chengcheng,et al
Natural Product-Inspired Discovery of Naphthoquinone-Furo-Piperidine Derivatives as Novel STAT3 Inhibitors for the Treatment of Triple-Negative Breast Cancer
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WB
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Human
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Citation
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Zhong W, Huang L, Lin Y, Xing C, Lu C
Endogenous dual miRNA-triggered dynamic assembly of DNA nanostructures for in-situ dual siRNA delivery
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WB
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Human
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Citation
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Mo, F., Zhou, X., Yang, M., Chen, L., Tang, Z., Wang, C., & Cui, Y.
Trehalose Attenuates Oxidative Stress and Endoplasmic Reticulum Stress-Mediated Apoptosis in IPEC-J2 Cells Subjected to Heat Stress
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WB
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Pig
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Citation
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Establishment and Characterization of a HER2-Positive Cell Line Derived from the Pleural Effusion of a Drug-Resistant Breast Cancer Patient
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WB
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Mouse
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Human
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Citation
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Liu Shengjie,et al
Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway
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WB
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Mouse
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Citation
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