c-Fos Recombinant Rabbit Monoclonal Antibody [JJ0938] (ET1701-95)

UNIPROT #

P01100

PROTEIN NAME

c-Fos

SYNONYMS

Activator protein 1 antibody;AP 1 antibody;C FOS antibody;Cellular oncogene c fos antibody;Cellular oncogene fos antibody;FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody;FBJ murine osteosarcoma viral oncogene homolog antibody;FBJ murine osteosarcoma viral v fos oncogene homolog antibody;FBJ Osteosarcoma Virus antibody;FOS antibody;FOS protein antibody;FOS_HUMAN antibody;G0 G1 switch regulatory protein 7 antibody;G0/G1 switch regulatory protein 7 antibody;G0S7 antibody;Oncogene FOS antibody;p55 antibody;proto oncogene c Fos antibody;Proto oncogene protein c fos antibody;Proto-oncogene c-Fos antibody;v fos FBJ murine osteosarcoma viral oncogene homolog antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family. Fos subfamily.

DEVELOPMENTAL STAGE

Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).; Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.; In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

SUBCELLULAR LOCATION

Nucleus, Endoplasmic reticulum, Cytoplasm.

FUNCTION

Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos.[5] It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus) (Curran and Tech, 1982). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2.It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression.It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.