Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [JM93-28] (ET1705-52)
Catalog# ET1705-52
Rabbit Monoclonal to Carcino Embryonic Antigen CEA
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
Lane 1: A549 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: BxPC-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 70-200 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [JM93-28] (ET1705-52)
Immunogen
Human carcinoembryonic antigen (cea) purified from human liver.
Host
Rabbit
Positive Control
A549 cell lysate, MCF7 cell lysate, BxPC-3 cell lysate, HUVEC, MCF-7, human lung cancer tissue, human stomach carcinoma tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM93-28
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 77 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Carcino Embryonic Antigen CEA
SYNONYMS
Carcinoembryonic antigen antibody;Carcinoembryonic antigen-related cell adhesion molecule 5 antibody;CD66e antibody;CEA antibody;Ceacam5 antibody;CEAM5_HUMAN antibody;DKFZp781M2392 antibody;Meconium antigen 100 antibody;OTTHUMP00000199032 antibody;OTTHUMP00000199033 antibody;OTTHUMP00000199034 antibody
SEQUENCE SIMILARITIES
Belongs to the immunoglobulin superfamily. CEA family.
TISSUE SPECIFICITY
Expressed in columnar epithelial and goblet cells of the colon (at protein level). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
POST-TRANSLATIONAL MODIFICATION
Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a member of the carcinoembryonic antigen (CEA) gene family. In the literature, CEACAM5 is often used as a synonym for cancer embryonic antigen (CEA), a well-known biomarker of many types of malignancies, colorectal cancer in the first place. Its primary function in the embryonic intestine and colon tumors is adhesion between epithelial cells. Also, it plays a significant role in the inhibition of differentiation and apoptosis in colon cells. There are evidences that high CEACAM5 expression is firmly associated with the CD133-positive colorectal cancer stem cells.
CITATIONS
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Wei-Gang Fang;Xin-Xia Tian
Extracellular ATP promotes breast cancer invasion and chemoresistance via SOX9 signaling
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WB
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IHC
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human
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Citation
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