Caveolin-1 Mouse Monoclonal Antibody [19-D6] (EM40728)
Catalog# EM40728
Mouse Monoclonal to Caveolin-1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of Caveolin-1 on different lysates with Mouse anti-Caveolin-1 antibody (EM40728) at 1/500 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: Lane 1: 3 minutes; Lane 2-4: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.