CD11b Mouse Monoclonal Antibody [4-6] (EM1701-42)
Catalog# EM1701-42
Mouse Monoclonal to CD11b
Tested for
-
IHC-P
-
IF-Cell
-
FC
-
WB
-
IF-Tissue
Reacts with
-
Human
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
