CD127 Recombinant Rabbit Monoclonal Antibody [JE37-10] (HA721214)
Catalog# HA721214
Rabbit Monoclonal to CD127
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of CD127 on K562 cell lysates with Rabbit anti-CD127 antibody (HA721214) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721214) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721214) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.