CD200 Recombinant Rabbit Monoclonal Antibody [PSH01-49] (HA721692)
Catalog# HA721692
Rabbit Monoclonal to CD200
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD200 on different lysates with Rabbit anti-CD200 antibody (HA721692) at 1/1,000 dilution.
Lane 1: SK-MEL-28 cell lysate
Lane 2: NCI-H226 cell lysate
Lane 3: Human brain tissue lysate
Lane 4: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40-50kDa
Exposure time: 2 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721692) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate
Lane 2: NCI-H226 cell lysate
Lane 3: Human brain tissue lysate
Lane 4: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 40-50kDa
Exposure time: 2 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721692) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.