CD204 Mouse Monoclonal Antibody [A8G2]
Catalog# HA601090
CD204 Mouse Monoclonal Antibody [A8G2]
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WB
-
IHC-P
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Human
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unconjugated
Overview
Product Name
CD204 Mouse Monoclonal Antibody [A8G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human CD204 aa 51-250 / 451.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 50 kDa
Positive Control
THP-1 cell lysate treated with 80nM PMA for 72 hours, human liver tissue.
Conjugation
unconjugated
Clone Number
A8G2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:5,000-1:10,000
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IHC-P
-
1:500
Target
Function
This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.
Background References
1. Tada Y et al. Infiltration of CD204-overexpressing Macrophages Contributes to the Progression of Stage II and III Colorectal Cancer. Anticancer Res. 2021 Oct
2. Nagano M et al. CD204-positive macrophages accumulate in breast cancer tumors with high levels of infiltrating lymphocytes and programmed death ligand-1 expression. Oncol Lett. 2021 Jan
Subcellular Location
Membrane.
UNIPROT
Synonyms
CD204 antibody
CD204 antigen antibody
CD24 antibody
Macrophage acetylated LDL receptor I and II antibody
Macrophage scavenger receptor 1 antibody
Macrophage scavenger receptor type III antibody
Macrophage scavenger receptor types I and II antibody
Msr 1 antibody
MSR1 antibody
MSRE_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA601090) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 cell lysate treated with 80nM PMA for 72 hours
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 75 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601090) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD204 antibody (HA601090) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD204 Recombinant Mouse Monoclonal Antibody [A8G2-R] - BSA and Azide free
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Rat
Conjugate: unconjugated
