CD21 Mouse Monoclonal Antibody [A4E5] (HA600001)
Catalog# HA600001
Mouse Monoclonal to CD21
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Western blot analysis of CD21 on different lysates with Mouse anti-CD21 antibody (HA600001) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 160 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 160 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.