CD35 Rabbit Polyclonal Antibody (ER2001-14)
Catalog# ER2001-14
Rabbit Polyclonal to CD35
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
Western blot analysis of CD35 on different lysates with Rabbit anti-CD35 antibody (ER2001-14) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 224 kDa
Observed band size: 280 kDa
Exposure time: 1 minute; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TF-1 cell lysate
Lane 2: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 224 kDa
Observed band size: 280 kDa
Exposure time: 1 minute; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.