CD39 Recombinant Rabbit Monoclonal Antibody [JA90-36] (ET1704-74)
Catalog# ET1704-74
Rabbit Monoclonal to CD39
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate (negative) (15 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Lane 3: Mouse placenta tissue lysate (20 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 70 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-74) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD39 Recombinant Rabbit Monoclonal Antibody [JA90-36] (ET1704-74)
Immunogen
Synthetic peptide within human cd39 aa 371-420 / 510.
Host
Rabbit
Positive Control
Mouse lung tissue lysate, mouse placenta tissue lysate, human tonsil tissue, human spleen tissue, human placenta tissue, mouse uterus tissue, human endometrium tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA90-36
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:50-1:400
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD39
SYNONYMS
ATPDase antibody;CD 39 antibody;CD39 antibody;CD39 antigen antibody;DKFZp686D194 antibody;DKFZp686I093 antibody;Ecto apyrase antibody;Ecto ATP diphosphohydrolase antibody;Ecto-apyrase antibody;Ecto-ATP diphosphohydrolase 1 antibody;Ecto-ATPase 1 antibody;Ecto-ATPDase 1 antibody;Ectonucleoside triphosphate diphosphohydrolase 1 antibody;ENTP1_HUMAN antibody;ENTPD 1 antibody;ENTPD1 antibody;FLJ40921 antibody;FLJ40959 antibody;Lymphoid cell activation antigen antibody;NTPDase 1 antibody;NTPDase1 antibody;SPG64 antibody
SEQUENCE SIMILARITIES
Belongs to the GDA1/CD39 NTPase family.
TISSUE SPECIFICITY
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.
POST-TRANSLATIONAL MODIFICATION
The N-terminus is blocked.; Palmitoylated in the N-terminal part.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENP1), is an integral membrane glycoprotein that acts as an extracellular nucleotide-hydrolyzing enzyme. CD39 inhibits ADP-induced platelet aggregation by hydrolyzing ADP to AMP and ultimately generating adenosine. Intracellular CD39 undergoes glycosylation at 6 N-glycosylation sites and translocates to the membrane in order to be an active enzyme. Alternative splicing gives rise to three CD39 isoforms, vascular, placenta I and placenta II. The placenta I isoform differs at the amino terminus whereas the placenta II isoform is missing amino acids 300-510 at the C-terminus. CD39 is expressed in vascular tissues including placenta, lung, skeletal muscle and kidney, as well as endothelium, smooth muscle, cardiac cells, lymphocytes (such as activated B cells) activated NK cells, macrophages, dendridic cells and platelets. CD39 may be used as an anti-thrombic agent for pre-treating patients at risk for coronary artery occlusion and thrombic stroke.
CITATIONS
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Yang Fan,et al
Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation
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WB
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Mouse
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Citation
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