CD48 Recombinant Rabbit Monoclonal Antibody [JE37-98] (HA721371)
Catalog# HA721371
Rabbit Monoclonal to CD48
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA721371) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: THP-1 cell lysate (negative)
Lane 4: K-562 cell lysate (negative)
Lane 5: Ramos cell lysate
Lane 6: Daudi cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 40-50 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA721371) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: THP-1 cell lysate (negative)
Lane 4: K-562 cell lysate (negative)
Lane 5: Ramos cell lysate
Lane 6: Daudi cell lysate
Lane 7: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 40-50 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.