Cdk2 Rabbit Polyclonal Antibody (R1309-3)
Catalog# R1309-3
Rabbit Polyclonal to Cdk2
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Positive control:
Lane 1: A549 cell lysate
Lane 2: F9 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Mouse thymus tissue lysate
Lane 5: Human liver tissue lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Cdk2 Rabbit Polyclonal Antibody (R1309-3)
Immunogen
Synthetic peptide within human cdk2 aa 249-298 / 298.
Host
Rabbit
Positive Control
A549 cell lysate, F9 cell lysate, PC-12 cell lysate, Mouse thymus tissue lysate, Human liver tissue lysate, A549, human tonsil tissue, human breast cancer tissue, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cdk2
SYNONYMS
Cdc2 related protein kinase antibody;cdc2-related protein kinase antibody;CDC28 antibody;CDC2A antibody;Cdk 2 antibody;CDK1 antibody;CDK2 antibody;CDK2_HUMAN antibody;CDKN2 antibody;Cell devision kinase 2 antibody;Cell division protein kinase 2 antibody;Cyclin dependent kinase 2 antibody;cyclin dependent kinase 2-alpha antibody;Cyclin-dependent kinase 2 antibody;kinase Cdc2 antibody;MPF antibody;p33 protein kinase antibody;p33(CDK2) antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.; Nitrosylated after treatment with nitric oxide (DETA-NO).
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cytoskeleton, Endosome.
FUNCTION
Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.
CITATIONS
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Leukemia inhibitory factor prevents chicken follicular atresia through PI3K/AKT and Stat3 signaling pathways.
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WB
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Chicken
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Citation
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Dong, J., Guo, C., Yang, Z., Wu, Y., & Zhang, C.
Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens
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WB
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Hen
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Citation
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Autophagy participates in germline cyst breakdown and follicular formation by modulating glycolysis switch via Akt signaling in newly-hatched chicken ovaries
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WB
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chicken
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Citation
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Chen, X., Huang, Y., Gao, P., Lv, Y., Jia, D., Sun, K., Han, Y., Hu, H., Tang, Z., Ren, X., & Liu, M.
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells
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WB
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Zebrafish
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Citation
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