CDKN1A/P21 Rabbit Polyclonal Antibody (HA500156)
Catalog# HA500156
Rabbit Polyclonal to CDKN1A/P21
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WB
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IHC-P
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Human
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Mouse
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 10μM NUTLIN 3A for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 21 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CDKN1A/P21 Rabbit Polyclonal Antibody (HA500156)
Immunogen
Recombinant protein within human cdkn1a/p21 aa 1-164.
Host
Rabbit
Positive Control
MCF7 treated with 10μM NUTLIN 3A for 24 hours cell lysate, U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, mouse colon tissue lysates, human esophagus tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 18 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CDKN1A/P21
SYNONYMS
CAP20 antibody;CDK-interacting protein 1 antibody;CDKI antibody;CDKN1 antibody;Cdkn1a antibody;CDN1A_HUMAN antibody;CIP1 antibody;Cyclin Dependent Kinase Inhibitor 1A antibody;Cyclin-dependent kinase inhibitor 1 antibody;Cyclin-dependent kinase inhibitor 1A (P21) antibody;Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody;DNA Synthesis Inhibitor antibody;MDA-6 antibody;MDA6 antibody;Melanoma differentiation-associated protein 6 antibody;Melanoma differentiation-associated protein antibody;p21 antibody;P21 protein antibody;p21CIP1 antibody;p21Cip1/Waf1 antibody;p21WAF antibody;PIC1 antibody;SDI1 antibody;SLC12A9 antibody;WAF1 antibody;Wild type p53 activated fragment 1 (WAF1) antibody;Wild type p53 activated fragment 1 antibody;Wildtype p53-activated fragment 1 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase. p21 interacts with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. However p21 may inhibit apoptosis and does not induce cell death on its own. The ability of p21 to inhibit apoptosis in response to replication fork stress has also been reported. Studies of p53 dependent cell cycle arrest in response to DNA damage identified p21 as the primary mediator of downstream cell cycle arrest. p21 is negatively regulated by ubiquitin ligases both over the course of the cell cycle and in response to DNA damage. Cytoplasmic p21 expression can be significantly correlated with lymph node metastasis, distant metastases, advanced TNM stage, depth of invasion and OS.
CITATIONS
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Wang Yaqin,et al
CISD2 downregulation participates in the ferroptosis process of human ovarian SKOV-3 cells through modulating the wild type p53-mediated GLS2/SAT1/SLC7A11 and Gpx4/TRF signaling pathway
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WB
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Human
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Citation
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Yang, L., Wang, B., Guo, F., Huang, R., Liang, Y., Li, L., Tao, S., Yin, T., Fu, P., & Ma, L.
FFAR4 improves the senescence of tubular epithelial cells by AMPK/SirT3 signaling in acute kidney injury
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WB
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Mouse
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Citation
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Zhao Jinquan,et al
The anti-non-small cell lung cancer effect of Diosbulbin B: targeting YY1 induced cell cycle arrest and apoptosis
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WB
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Human
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Citation
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TPP1 inhibits DNA damage response and chemosensitivity in esophageal cancer
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WB
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Human
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Citation
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