Cofilin Rabbit Polyclonal Antibody (HA500523)
Catalog# HA500523
Rabbit Polyclonal to Cofilin
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (HA500523) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: MDA-MB-468 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: HUVEC cell lysate
Lane 7: Jurkat cell lysate
Lane 8: COS-1 cell lysate
Lane 9: VERO cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: PC-12 cell lysate
Lane 12: Neuro-2a cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500523) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: MDA-MB-468 cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: HUVEC cell lysate
Lane 7: Jurkat cell lysate
Lane 8: COS-1 cell lysate
Lane 9: VERO cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: PC-12 cell lysate
Lane 12: Neuro-2a cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500523) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.